Tuesday, September 10, 2019

DNA Barcoding Lab Report Example | Topics and Well Written Essays - 750 words

DNA Barcoding - Lab Report Example The protocol utilizes short sequences of organisms to characterize them. These oligonucleotides fall in positions in the genome which are agreed upon and its standard for a particular genome of interest. The DNA barcode sequences are also rather short in comparison to the entire genome and can be extracted with relative ease utilizing cheap methods. For instance, the cytochrome C oxidase subunit 1 mitochondria region (COI) has in the recent times being the standard barcode region for higher animals. One define characteristic of the DNA barcode is its commonality within a species (within species) and variations among species (without species). ie for a selected DNA barcode of a particular species there exists ranging differences and these differences are minor in individuals of the same species to gurantee the sequence segment to be used as a barcode. In obtaining the DNA for branding, the mayfly should be killed in a ‘DNA friendly fashion’ by avoiding the use of preservation agents such formalin which may degrade DNA. Genomic DNA is isolated via the fast DNA extraction method from fresh or frozen specimens. Combination of Chelex protocol with Proteinase K may rule out the need for tissue disruption while guaranteeing the release of DNA leaving chitinous material left intact. PCR amplification is done with an optimal primer specific for the barcode region. The barcode products obtained from the PCR are in most instances sequences bidirectional and later deposited in the barcode reference library.  

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